e coli atcc Search Results


pcw7  (ATCC)
90
ATCC pcw7
Pcw7, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli j5 lps  (ATCC)
92
ATCC e coli j5 lps
SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— <t>E.</t> <t>coli</t> 0119; 6— E. coli <t>J5;</t> 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; <t>11—LPS-MW</t> Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.
E Coli J5 Lps, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid pbrsv  (ATCC)
92
ATCC plasmid pbrsv
SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— <t>E.</t> <t>coli</t> 0119; 6— E. coli <t>J5;</t> 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; <t>11—LPS-MW</t> Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.
Plasmid Pbrsv, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli  (ATCC)
90
ATCC e coli
SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— <t>E.</t> <t>coli</t> 0119; 6— E. coli <t>J5;</t> 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; <t>11—LPS-MW</t> Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.
E Coli, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid prs314  (ATCC)
91
ATCC plasmid prs314
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Plasmid Prs314, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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length hpv type 16 virus  (ATCC)
94
ATCC length hpv type 16 virus
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Length Hpv Type 16 Virus, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tev protease  (ATCC)
90
ATCC tev protease
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Tev Protease, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atcc no 77107  (ATCC)
93
ATCC atcc no 77107
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Atcc No 77107, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trp1  (ATCC)
91
ATCC trp1
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Trp1, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pre107  (ATCC)
90
ATCC pre107
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Pre107, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli strain bl21  (ATCC)
90
ATCC e coli strain bl21
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
E Coli Strain Bl21, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ptr uf11 plasmid  (ATCC)
94
ATCC ptr uf11 plasmid
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Ptr Uf11 Plasmid, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— E. coli 0119; 6— E. coli J5; 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; 11—LPS-MW Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.

Journal: International Journal of Molecular Sciences

Article Title: Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification †

doi: 10.3390/ijms25073927

Figure Lengend Snippet: SDS-PAGE analysis of the different LPSs used in the present study. 1— Y. pestis ; 2— Y. enterocolitica ; 3— B. pertussis 1414; 4— B. pertussis A100; 5— E. coli 0119; 6— E. coli J5; 7— N. meningitidis ; 8— P. aeruginosa P3; 9— S. Typhimurium Ra; 10— R. pickettii ; 11—LPS-MW Standard. Staining was performed according to Tsai and Frash . Rough-type LPSs migrate in the low-masses level at about 4 kDa.

Article Snippet: E. coli J5 LPS was extracted from strain ATCC 43745.

Techniques: SDS Page, Staining

MALDI mass spectra obtained for tetra-acyl E. coli J5 lipid A ( A ) and hexa-acyl E. coli J5 lipid A ( B ). Corresponding lipid A structures ( C ). Comparison of LAL and HEK-blue hTLR-4 activities ( D ). Fatty acids other than 14:0-3-OH are presented with different colors.

Journal: International Journal of Molecular Sciences

Article Title: Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification †

doi: 10.3390/ijms25073927

Figure Lengend Snippet: MALDI mass spectra obtained for tetra-acyl E. coli J5 lipid A ( A ) and hexa-acyl E. coli J5 lipid A ( B ). Corresponding lipid A structures ( C ). Comparison of LAL and HEK-blue hTLR-4 activities ( D ). Fatty acids other than 14:0-3-OH are presented with different colors.

Article Snippet: E. coli J5 LPS was extracted from strain ATCC 43745.

Techniques: Comparison

Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain BJ5464+pRS314-β were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.

Journal:

Article Title: PDI Improves Secretion of Redox-Inactive β-glucosidase

doi: 10.1021/bp060287p

Figure Lengend Snippet: Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain BJ5464+pRS314-β were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.

Article Snippet: Plasmids The single copy expression plasmid pRS314-β was constructed from a secretion-modified full-length β-glucosidase with an N-terminal synthetic pre-pro secretion signal sequence and a 10-amino-acid C-terminal c- myc detection tag under the control of a GAL1-10 promoter and the plasmid pRS314 (ATCC) containing the TRP1 marker as reported previously 5 .

Techniques: Comparison, Expressing, Western Blot, Software